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However, it is currently unclear whether there are other explanations as to why Rac2 is more important for NADPH oxidase activation or whether its RQQKRP sequence can influence any other p67 phox -independent downstream pathways

Glutamine utilization by rat neutrophils: presence of phosphate-dependent glutaminase

Nephrology, 17 (4), 311-321

doi: 10.3390/nu12102929

For the neutral conditions, the slides were equilibrated for 20 min in an ice-cold buffer containing 100 mM Tris and 300 mM sodium acetate, with pH adjusted to 9.0 using glacial acetic acid